Journal: PLoS ONE

Article Title: A Single Intramuscular Vaccination of Mice with the HSV-1 VC2 Virus with Mutations in the Glycoprotein K and the Membrane Protein UL20 Confers Full Protection against Lethal Intravaginal Challenge with Virulent HSV-1 and HSV-2 Strains

doi: 10.1371/journal.pone.0109890

Figure Lengend Snippet: (A) Colorimetric ELISA based analysis of HSV-1 reactive polyclonal IgG produced 21 days post vaccination n = 20. Statistical comparison conducted by SAS using the T test Procedure. Bars represent the 95% confidence interval about the mean (B) Titration of serum neutralizing fixed PFU of HSV-1 (McKrae) normalized to a no serum control n = 5. Significant reduction in PFU observed 1∶160, 1∶80, 1∶40, and 1∶20 dilutions of the sera. Statistical comparison conducted by SAS using The Mixed Procedure and Differences in Least Squares Means. Bars represent the 95% confidence interval about the mean. (C) Cross reactive neutralization of HSV-1 (McKrae) and HSV-2 (G) at a 1∶20 dilution of sera from vaccinated and mock inoculated mice. Percent neutralization normalized to no serum controls. Statistical comparison conducted by SAS using The Mixed Procedure and Differences in Least Squares Means. Bars represent the 95% confidence interval about the mean. Significant differences noted as * p≤0.05, ** p≤0.01, or *** p≤0.0001.

Article Snippet: Labeled cells were then cultured at a concentration of 10 5 cells/well in a 96 well U-bottom plate and incubated at 37°C and 5% CO 2 for 7 days in the presence of pooled peptides specific to either HSV-1 or HSV-2 at a concentration of 10 µg/mL Cells were then stained with polyclonal anti-mouse CD4 antibody conjugated to PE (BD Biosciences) and polyclonal anti-mouse CD8a antibody conjugated to APC (BD Biosciences).

Techniques: Enzyme-linked Immunosorbent Assay, Produced, Titration, Neutralization